Journal: FEBS Open Bio
Article Title: Intracellular protein crystallization in living insect cells
doi: 10.1002/2211-5463.70020
Figure Lengend Snippet: Laboratory setup for MicroMesh loading. (A) A standard upright cell culture light microscope is equipped with a custom‐made holding device for a MicroMesh mounted on a magnetic goniometer base. A continuous humid air stream to keep the cells hydrated during loading is produced by passing compressed air through different water‐filled bottles (left). An empty bottle at the end prevents sample contamination with water droplets. (B) and (C) After adjusting the MeshMount in the optical focus of the microscope, the cells are directly pipetted onto the surface, followed by the removal of the excess liquid using an extra fine liquid wick from the back of the mesh. Immediately after applying cryo protection by pipetting 40% PEG200 on the loaded cells and blotting of exceeding liquids, the MeshMount is flash‐frozen in liquid N 2 .
Article Snippet: Extra fine liquid wicks (MiTeGen #W‐XF).
Techniques: Cell Culture, Light Microscopy, Produced, Microscopy